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Finally, gels were incubated in destaining/fixing solution [methanol:acetic acid:water (30:10:60)], then stained with 0.1% amido black in 10% acetic acid for 1 h and destained successively in destaining/fixing solution, 10% acetic acid, and 1% acetic acid, which revealed clear zones of substrate hydrolysis on a blue background

In a group of deficient patients who were treated and followed up for 224 months, the patients with stomatitis showed a complete recovery [4]
The same principle applies with even more urgency in a combination protocol

doi: 10.3390/antiox12101850, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 253
They increase the expression of beta-adrenergic receptors, leading to enhanced cardiac output
