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Age is not a restriction, as changes in location are common in both young and adult animals

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Chorioallantoic angiogenesis membrane assay (CAM) Fertilized chicken eggs (purchased from China Agriculture University) were incubated for 6 days after breeding at 37 C with 60% humidity
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the catheters were flushed with heparinized saline before and after blood sample collection and filled with heparin-glycerol locking solution (6 mL glycerol, 3 mL saline, 0.5 mL gentamycin (lOOmg/mL), 0.5 mL heparin (10,000 u/mL)) when not used for more than 8 h
