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Notably, analgesic effects have also been found in the mouse hot plate tests, where GHK reduced paw-licking reflexes, indicating pain-relieving activity at very low doses (0.5 g/kg) [239], though detailed mechanistic descriptions still remain scarce

Finally, the pellet was resuspended in 2 ml of PBS, and 500 l of elution buffer (50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0) were added to elute the bound E