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All steps were the same as for the 96-well plate assay except that the culture volume was 1 mL, the volumes of the fixative, staining solution, and acetic acid were scaled proportionally (0.5 mL fixative, 1.25 mL of crystal violet solution, and 1.25 mL of glacial acetic acid per tube), and the washes were performed by gentle pipetting (2 mL water per tube per wash) instead of by submersion

10.1016/s0021-9258(18)53184-7 88 SmithA.RishK

Current areas of scientific investigation include extracellular matrix signaling analysis, peptide-metal coordination studies, redox-sensitive signaling pathway research, transcription-associated metal regulation, copper-mediated cellular signaling investigations, and matrix-associated biochemical pathway analysis

Side effects are rare and mild, usually limited to brief muscle soreness, local discomfort, or dizziness

Dong Y, Jiang H (2016) Microbial production of metabolites and associated enzymatic reactions under high pressure

The application of prebiotics can prevent the adherence of pathogenic organisms to the epithelial cells of the fish gut, remove harmful bacteria from the gut, control inflammation and act as modulators of the host immune system directly and indirectly (Gephart et al., 2020)