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Approximately 75% of human genome is transcribed into RNAs [ FIGURE 1 Manufacturing exogenous mRNA has several benefits, primarily being more convenient, cost-effective, and final end-products not being subjected to additional regulations and scrutiny associated with genetically modified organisms [ In fact, one of the most well-known mRNA vaccines against SARS-CoV-2 has a current count of more than 13.72 billion doses administered worldwide [ mRNA design and modifications mRNA is a polymer of ribonucleotide building blocks, each composed of a ribose sugar, a phosphate group, and a nitrogenous base (adenine, guanine, uracil, or cytosine) [Figure 2) [ FIGURE 2 Nucleotide modification Nucleotides govern the dynamics of the expression of the genetic information encoded in the mRNA and they can be modified enzymatically or non-enzymatical (alkylation, oxidation or UV damage), to alter the biological properties of mRNA

Cur Drug Disc Technol 20:7488

Genis-Mendoza, Alma Delia, et al

10.1016/j.cell.2017.10.036 23

Remarkably, the charge distribution and shape of TrxR1 in this site is different with respect to TGR 33 due to the presence of charged and bulky residues, i.e., E337, D338, E341, E368 and K389 in human TrxR1 in place of A436, G437, Q440, S467 and D488 in TGR

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