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Founder mice with the modified Dmrta2 2xHA allele were screened for appropriate integration of the transgenic sequences at the knock-in region as well as for the presence of predicted ( potential off-targets followed by PCR followed by Sanger sequencing of the PCR products (Genewiz)

Also, it was shown that under water stress major biochemical and physiological changes took place, e.g

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Int J Curr Microbiol App Sci 5(12):678686 Shin YE, Hwang IW, Jin HJ (2016) Association between glutathione S-transferases M1, T1 and P1 gene polymorphisms and prostate cancer in Koreans