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Reactions were carried out on the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems)

A., Iype, S

As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins

However, whether a similar p53SLC7A11 regulatory mechanism contributes to ferroptosis susceptibility in OA chondrocytes remains unclear and requires further investigation (85)

Recognizing its symptoms and implementing effective recovery strategies is crucial

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