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(1050) David, L

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The supernatant was collected and used further for the determination of glutathione using Ellmans reagent (5,5'-dithiobis-2-nitrobenzoic acid, DTNB) by spectrophotometry, since the DTNB reduction product has a strong absorption at 412 nm

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The reason for occurring this phenomenon (reduction of CySGly- but not of GS-bound Mercury(II)) in solution and in the biological environment of cells is reasonably a consequence of the low reducing electrochemical potential of CySGly (at 0.282 V the most reducing one of the whole soluble physiological thiolome)

In the nucleus, Nrf2 induces the expression of cytoprotective genes that mitigate ferroptosis by regulating iron metabolism and enhancing antioxidant defenses
