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New -substituted succinate-based hydroxamic acids as TNF- convertase inhibitors

Animals, orthotopic tumor implantation, and diet allocation Cell implantation Mice were anesthetized with Ketamine/Xylazine (100 mg/kg and 10 mg/kg, respectively) and assessed for lack of reflexes by toe pinch

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Pelleted cells were lysed with lysis buffer (B-PER Bacterial Protein Extraction Reagent, ThermoFisher), lysozyme (100 g/ml), PMSF (1 mM, Sigma), protein inhibitor cocktail (ThermoFisher), DNAse (5U/ml), at room temperature for 15 min, then on ice for 15 min, then spun for 30 min
