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Immunofluorescence Fibroblast cultures at passage 3 were cultured in eight-well chamber slides (Gibco, Burlington, ON, Canada) in growth medium (DMEM, 10% fetal bovine serum (FBS), penicillin, and streptavidin from Invitrogen, Burlington, ON, Canada)

5.3 Cytokines and macrophage polarization Ferroptosis-driven inflammation promotes the polarization of macrophages toward an M2-like, pro-fibrotic phenotype, largely mediated by cytokines such as TGF- and IL-10 (164)

20 l aliquots (whole cellular protein sample) are transferred to a PCR microplate containing 7 l of 4xSDS-PAGE sample buffer

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Grunau, B

Stage II begins with the onset of liver injury, generally within 24 hours but always within 36 hours of ingestion (14)