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Pharmacokinetic and pharmacodynamic modeling of an anti-interleukin-6 chimeric monoclonal antibody (siltuximab) in patients with metastatic renal cell carcinoma

doi: 10.1186/s13024-015-0038-3

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Immunoprecipitated chromatin was eluted with the ChIP direct elution buffer (0.5% SDS, 5 mM EDTA, 10 mM Tris-HCl [pH 8], 300 mM NaCl) and incubated at 65 C for 4 h to reverse the crosslinks

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