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For immunoblotting, membranes were blocked in 5% skim milk powder and 1% BSA in PBST for 1 h and incubated in anti-5-hydroxymethylcytosine antibody

[PMC free article: PMC1478898] [PubMed: 2097025] 95

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However, there is no strong evidence in this area, recommending further investigations to clarify the possible adverse effects of riboflavin intake in high amounts

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J Biol Inorg Chem 16:381390
