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The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins

[DOI] [PubMed] [Google Scholar] 227.Wang SJ, Birtles S, de Schoolmeester J, et al

Gathering progress notes, lab results, imaging reports, and medical necessity letters from the ordering physician