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The development of follicles from the primordial stage to antral follicles is accompanied by a marked increase in the metabolic function of granulosa cells, especially a large increase in cytochrome P450 activity with steroid biosynthesis [36]

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Although the Dietary Reference Intakes for Calcium and Vitamin D report issued in 2011 by the Institute of Medicine proposes 20 ng/mL as the definition of sufficiency based solely on requirements to prevent osteoporosis 14 , it has been hypothesized that serum 25(OH)D concentrations of 3032 ng/mL (7580 nmol/L) are optimal in healthy populations 1517