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As a relatively weak pathogen, MP acts to ignite a pre-existing immunological powder keg. Genome-wide association studies have linked specific HLA genotypes and polymorphisms in innate immune signaling molecules (TLRs and IL-1R) to increased susceptibility to RMPP (153)

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Anti-inflammatory reflex action of splanchnic sympathetic nerves is distributed across abdominal organs

Elution can then release the purified protein by simply changing the buffer conditions, for example in a shift in pH, ionic strength, or molarity, or by using a competitive ligand

Additionally, folate, iron, and thyroid function should be evaluated, as deficiencies in these nutrients interact with B12 metabolism and can mask or amplify symptoms