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While resistant cells upregulate the System Xc - -GSH-GPX4 axis to counteract oxidative stress (e.g., SLC7A11 overexpression in lung cancer), this defense is inherently fragile ( - activity is suppressed by CD8 + T cell-derived IFN (via JAK/STAT-mediated SLC7A11 downregulation), and (46)

There are a number of reasons that could attribute to this, mainly the low concentration of protein in the cell lysate used for each reaction

doi: 10.1001/jamaneurol.2014.3625

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[2] The most common adverse reaction to parenteral thiamine administration is an injection site reaction