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Ferroptosis can be induced by genetically deleting GPX4 or by using small molecules that either covalently inhibit GPX4 (e.g., RSL3) or promote its degradation (e.g., FIN56) [33, 39, 40]

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Lee I, Bender E, Arnold S, Kadenbach B

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Add labeled ligand to the beads, and measure how much ligand is left in the supernatant (not bound to the beads)

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