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To verify the health status of the rats before and after administration, blood samples were collected at 0 and 24 h after administration of test substances and sent to the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University for blood tests to determine creatinine, alanine transaminase (ALT), and aspartate transaminase (AST) with normal range 0.20.7 mg/dL, 1356 U/L, and 34109 U/L, respectively

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Ethics declarations Competing interests M.J.D has acted as a consultant, advisory board member and speaker for Boehringer Ingelheim, Eli Lilly, Novo Nordisk and Sanofi

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Two mutations occurred in the 3 untranslated region of an alternative splice form of HGF, while the third mutation altered splicing by affecting the relative strengths of the spliced forms of HGF gene

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