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Pathak treated an atherosclerotic mouse model with iodomethylcholine (IMC), a mechanism-based inhibitor of choline TMA lyase, and the loss of neutral sterols in mice feces was identified in the form of coprostanol (a bacterial metabolite of cholesterol) (83)

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10.1002/1097-4644(20010101)80:1 3.0.CO;2-W

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As the parameters measured did not differ significantly between the two LC doses used, the study concluded that a low dose of LC was sufficient to render positive effects on pregnancy and offspring outcomes in pregnant mice [42]