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Riboflavin is essential for homocysteine metabolism, acting as a cofactor for enzymes like methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR)
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To induce genome editing, cells containing both enzyme and gRNA plasmids were first cultured in SCLeuUra medium with 2% glucose at 30 C until saturation
