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10.3390/cells9092125 8 BrugueraE
& Sailor, M
After washing with 1 ml PBS solution, cells were further crosslinked with 3 mM disuccinimidyl glutarate (Fisher Scientific) in 2.5 ml PBS solution at 30 C for 40 min
hormonal confirmation), and confounding dietary or behavioral factors
As stated above, ROS levels are increased in cochlear HCs exposed to excessive noise, either indirectly via NADPH oxidase and MAPK signaling cascades or directly via changes to cell morphology and the cell cycle, programmed death, and other physiological processes
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