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Meanwhile, the challenges in generating non-NF1 pLGG models include the low proliferation capacity and the specific permissive tumor microenvironment necessary for glioma formation ( NF1 heterozygous cells along with multiple different types of cells in the microenvironment to grow (77, 8791, 93), whereas NF1 and non-NF1-pLGG xenografts needed loss of specific T-cell population to form (68)

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UC-MSCs were cultured in -MEM (Gibco, Grand Island, NY, USA) with 1% antibioticantimycotic solution (Gibco) and 10% fetal bovine serum

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