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After washing 2 times with cold-PBS solution and fixation with 4% paraformaldehyde, cells were permeabilized with 0.3% Triton X-100 PBS solution, blocked with 0.1% Tween-20 PBS solution (PBST) containing 2% bovine serum albumin (BSA), and immunostained with total MyHC (MyHC-T) antibody (supplementary Table S2) overnight at 4

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Multiple Nutritional Factors and Thyroid Disease, with Particular Reference to Autoimmune Thyroid Disease

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Reports using continuous beds in affinity chromatography have shown that the efficiency of these materials is as good as that for particle-based supports