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After 30 minutes of antibiotic treatment at 37C, the cells were washed three times with PBS

Eldridge S, Guo L, Hamre J

The skins outermost layer repels water-loving molecules like L-ascorbic acid and that means that the molecule may not get sufficiently absorbed, Dr
Crumb & Clarkson, 1992
A quality-of-life study in healthy adults supplemented with Lentinex beta-glucan of shiitake culinary-medicinal mushroom, Lentinus edodes (Agaricomycetes)

PMID: 21265979