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The K907A mutant IRE1 expression plasmid was generated through site-directed mutagenesis using the pLEX-hIRE1 plasmid (pLEX-hIRE1-K907A)

see Table 1) ( TABLE 1 Vitamin B12 Adequate Intake [AI] across life course ( Comparable age groupings are proposed in the Dietary Guidelines for Americans, though the recommended intake levels differ, beginning at 0.5 g in infancy, increasing to 2.4 g for adults, and rising modestly during pregnancy and lactation (2.6 g and 2.8 g, respectively

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Lei S, Su W, Xia ZY, Wang Y, Zhou L, Qiao S, Zhao B, Xia Z, Irwin MG