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Although there are other pathways for the generation of NADPH that can be used in the regulation of the red-ox state of cells, the PPP represents the major and most important pathway for NADPH production, especially in erythrocytes

Healthy cells maintain a ratio of 100:1 or higher (99% GSH, 100:1 The reality is that glutathione declines with age-by age 60, levels can be 30-50% lower than in youth

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The same microscope settings (illumination, exposure time, laser intensity) were used for all conditions and genotypes throughout one experiment