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Proceedings of the National Academy of Sciences, 86: 63776381
10.1038/gene.2015.18 68
National Natural Science Foundation of China (82405210)
However, when using Dynabeads magnetic beads for activation of T cells, no ligand for CTLA-4 (B7) is present, so T cell expansion is not inhibited
Lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes (Amersham Protran, Cytiva), and probed with antibodies
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