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Description
The samples were shaken for 1 hour at RT after adding 5 mL of HPLC-grade tert -butyl methyl ether, anhydrous, 99.8% (Sigma-Aldrich, 34875-2L)
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2F and Supplementary Data 1A)
Cytotoxicity was calculated as a percentage relative to untreated controls, and IC50 values were determined by fitting dose-response curves using nonlinear regression analysis in GraphPad Prism
The targeted analysis of succinate and glutarate was always paired, as both metabolites could be detected by their distinct RT
