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The chemical reaction involves the deprotonation of C4 in the A ring of the steroid substrate, thereby enabling the double bond between C5 and C6 to isomerize to a double bond between C4 and C5 (Figure 2)

After stimulation, cells were washed once with 1x PBS and then collected in 150 l 2x Laemmli Sample Buffer supplemented with 5% 2-Mercaptoethanol

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Sousa AR, Parikh A, Scadding G: Leukotriene-receptor expression on nasal mucosal inflammatory cells in aspirin-sensitive rhinosinusitis

In CMT4B3 fibroblasts, Myo-Ins did not improve any respiratory parameter, consistent with the requirement for intact mitochondrial function and PIP signalling to support Myo-Insmediated enhancement of mitochondrial plasticity