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collagenase solution DS with 0.1% (wt/vol) type 2 collagenase and 96 g/mL soybean trypsin inhibitor the proximal tubule suspension was centrifuged for 5 min at 170 g, washed, and then resuspended into the appropriate amount of culture medium (1:1 DMEM/F12 without phenol red and supplemented with heat-inactivated 1% FCS, 15 mmol/L Hepes, 2 mmol/L L-glutamine, 50 nmol/L hydrocortisone, 5 g/mL insulin, 5 g/mL transferrin, 5 ng/mL sodium selenite, 0.55 mmol/L sodium pyruvate, 10 mL/L 100 nonessential amino acids, 100 IU/mL penicillin, and 100 g/mL streptomycin buffered to pH 7.4 (osmolality of 325 mOsmol/kg H 2 O)

2014 Nov 20;9(11):e110598

DOI: Published: Publisher Name: Springer, Cham Print ISBN: 978-3-030-67726-8 Online ISBN: 978-3-030-67727-5 eBook Packages: MedicineMedicine (R0)

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A final proof by BPC 157 therapy (not achievable by dopamine agents) is the counteraction of the dopamine receptor supersensitivity (and thereby counteraction of amphetamine over-activity in haloperidol pretreated mice) as a general phenomenon given that counteraction confirms the resolution of the otherwise determining issue of decreased dopamine synthesis [152,164]
