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Description
EGFR is overexpressed by a number of TNBC cells such as MDA-MB-468 (obtained from primary tumor source) and MDA-MB-231 (from metastasis cancer tissue) 13,14

After blocking, they were processed with specific primary antibodies at 4 C overnight and with secondary antibodies in next day

Like conventional glyoxalase enzymes (GLYI + GLYII), different physio-chemical properties of unique glyoxalase III proteins were analyzed

Other activators of the kinase are potassium and magnesium ions
Whether this is due to intake of 2-methylfuran via food or potential endogenous formation is currently unclear

The experiment met the requirements of animal ethics with IRB no
