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The tissue samples were minced into small fragments, rinsed with ice-cold PBS, and homogenized using a tissue homogenizer until no visible solid particles remained (or alternatively ground in liquid nitrogen)

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Net Capital Gain/Loss: The total of all gains and losses after offsetting gains with losses

These are theoretical concerns, and real-world clinical experience has not shown clinically dangerous absorption changes in most patients

Smith MG, Des Etages SG, Snyder M

Superoxide and peroxynitrite generation from inducible nitric oxide synthase in macrophages