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Serine and its derivative glycine (via SHMT1/2) fuel nucleotide, protein, and lipid biosynthesis, antioxidant defenses (including glutathione and NADPH production), and one-carbon metabolism for methylation reactions [14]
Protein staining in the gel was performed using a Coomassie staining solution (Ammonium sulfate 10%phosphoric acid 10%Coomassie G250 0.12% and ethanol 20%) overnight with shaking at room temperature followed by destaining with distilled H 2 O

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Push the plunger slowly to administer the full dose
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