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To stop enzymatic activity, the cell suspension was washed again with cold DMEM/F12 and centrifuged (5 min, 4 C, 300 g )

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Sequencing was performed on an Illumina novaseq 6,000 instrument with 150 bp paired end reads

Janak A, Peacock O, White M, Guraieb-Trueba M, Nguyen ST, Alshenaifi J, Kopetz S , Johnson B, Smith GL, Chang GJ, You Y