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Nurse practitioners can prescribe independently in some states, or under physician supervision in others

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IHC was performed by thawing the slides (all steps were performed at RT unless otherwise indicated) in a humidifying tray for 5 min, permeabilized for 15 min with 1 PBS + 0.1% Tween (PBST), and blocked in blocking buffer (PBST + 1% normal donkey serum (NDS)) for 30 min

The LPC/MS unique genes were enriched for lysosomal biogenesis and lipid degradation programs ( GPNMB, NPC2, GALC, CTSZ, TPP1, IGF2R ), consistent with active phagocytosis of myelin debris, whereas the CPZ-specific genes suggest a chronic stress and metabolic reprogramming response ( CRYAB, CPT1A, FGR, CDKN2B, GPX3)
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