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While this purification is conceptually very similar to many other types of affinity purifications, there are a few specific things to keep in mind when purifying GST-fusion proteins

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A decrease in the expression of the pro-ferroptotic proteins ACSL4, TFR1, and VDAC2/3 and a decrease in dihydroethidium (DHE) fluorescence, iron, malondialdehyde (MDA), and myeloperoxidase (MPO) were further observed in metformin-treated mice

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