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Use the peptide mass listed on the Certificate of Analysis to calculate required solvent volume

Gastroenterology 155 , 10171021 (2018)

Ibanez-CervantesGCruz-CruzCDuran-ManuelEMLoyola-CruzMACureno-DiazMACastro-EscarpulliGet al

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This disciplined, precise approach is what separates anecdotal observation from rigorous science

doi: 10.1021/bi00414a064