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Santer FR, Malinowska K, Culig Z, et al

For tyrosinase activity, the cells were lysed in 100 L of 50 mM sodium phosphate buffer (pH 6.5) containing 5 L of 1% Triton X-100 and 5 L of 0.1 mM phenylmethylsulfonylfluoride

This can be explained by feedback inhibition mechanisms which prevent excessive accumulation of discrete metabolites and preserve homeostasis (Naz et al

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There is documentation of nicotine-induced inhibition of myofibroblast differentiation in human gingival fibroblasts in vitro 46