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Indirect calorimetry and metabolic studies Body composition was assessed using an EchoMRI-100 T system (Echo Medical Systems) in multiple cohorts of Gpr146-deficient mice, including whole-body knockout ( Gpr146/ ) and control ( Gpr146/ ) mice (both sexes) fed either chow or high-fat diet (HFD), as well as adipose-specific knockout mice subjected to HFD feeding

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These workflows commonly involve repeat experiments and cross-operator execution, which increases the value of a stable reference record and consistent identity fields

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