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For the electron coupling assays, respiratory substrates (10 mM pyruvate + 2 mM malate, 10 mM succinate + 2 M rotenone, 80 M palmitoyl-carnitine or octanoyl-carnitine + 0.5 mM malate or 10 mM glutamate + 10 mM malate) were diluted in MAS buffer with BSA (70 mM sucrose, 220 mM mannitol, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES and 1 mM EGTA, pH 7.2, in 0.2% FA-free BSA) and added to mitochondria

10.1038/s41586-020-2971-8 75

After weighing, fresh thalli were immediately frozen in liquid nitrogen

It creates a toxic environment that gums up the works, impairs communication between neurons, and drains your resources, directly contributing to that foggy feeling and accelerating the ageing process

For collagen culture (Fig

Heart tissues isolated from FLAG-BNIP3 mice were initially washed with PBS, dissected, and homogenised in immunoprecipitation (IP) lysis buffer composed of 1 Cell Lysis Buffer (Cell Signaling), 100 mM PMSF (Roche), and 1 protease and phosphatase inhibitor cocktail (ThermoFisher)