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GSTs were purified from frozen plant tissue using 4 v/w 100 mM Tris-Cl pH 7.5 containing 150 mM NaCl, 1 mM EDTA, 50 g/ml avidin, 10 g.ml -1 bovine pancreatic DNase I, 10 g.ml -1 bovine pancreatic RNase A and 5% w/v insoluble polyvinylpolypyrrolidone

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TRPA1, transient receptor potential ankyrin 1