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sgRNA expressing vector along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK-293T cells using the XTremeGene 9 transfection reagent (Roche)

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In the case of multiple myeloma, these abnormally elevated SAMe levels have been linked to high MAT2A expression, and inhibiting it leads to SAMe levels decrease and impaired cell viability and proliferation [237]