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Description
Membranes were blocked in 5% bovine serum albumin with 0.1% Tween 20 for one hour and then incubated with primary antibodies against TNF- (1:1500, KRC3011, Invitrogen, USA), Bcl-2 Associated X-protein (BAX) (1:1000, BS-28034R, Bioss, USA), Bcl-2 (1:1000, MAB8272, R&D systems, USA), p-S2448-mTOR (1:2000, ab109268, Abcam, USA), p-S473-Akt (1:2000, ab8805, Abcam, USA), and -actin (1:2500, Abcam, USA)

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Zeroshot kafein ierii ile, geceleri uzun saatler verimli bir ekilde ders almak isteyenler iin pratik bir alternatiftir

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