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Spleen and lung RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-BM12 mice (Mut/Mut), then cDNA libraries were synthesized by reverse transcription, followed by PCR with H2-Ab1 primers

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After introduction of animal food, the cobalamin level increases and peaks at 37 years and then decreases, median plasma tHcy decreases and remains low until 7 years when it starts increasing, whereas median plasma MMA decreases after 12 months and remains low throughout childhood (Monsen et al., 2003)

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However, full-thickness tears where the tendon has completely detached from bone typically require surgical reattachment