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CCK-8 assay Irradiated or non-irradiated Hepa1-6 cells were seeded in 96-well plates at a density of 3 10 4 cells per well and incubated at 37 C in 5% CO 2 incubator

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[24] demonstrated high ASCT2 expression in the gastric tissues of patients with GC

However, the increased levels of 4HNE in hippocampal homogenates (which aligns with increases we previously reported in cortical homogenates 82 ) but only trend to increase in CA1 4HNE-IR suggests that the major source of 4HNE may not be the neuronal cell body

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