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[DOI] [PubMed] [Google Scholar] 75.Smallman Pahor A

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Several targeted strain strategies have been developed through the introduction of DNA mutations that affect protein synthesis, degradation, secretion, or folding (reviewed in Makino et al., 2011), including: engineered strains for improved protein processing at low temperatures, such as the Arctic Express strain (Agilent Technologies)

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Cas9 mRNA, gRNA, and the donor vector were microinjected into fertilized eggs of the C57BL/6J mice to generate the F0 (chimera) generation