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For IFN- and Foxp3 staining, cells were first cultured with phorbol 12-myristate 13-acetate (PMA, sc-3576, Santa Cruz Biotechnology), ionomycin (ALX-450-007, ALEXIS Biochemicals) and brefeldin A (423303, BioLegend) for 6 h, and followed by intracellular staining using an intracellular staining kit (554714, BD Biosciences)

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Astroglial pathophysiology can be broadly classified into: (1) astroglial reactivity or reactive astrogliosis