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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel

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Mol Asp Med 24:293303 Wang X, Michaelis EK (2010) Selective neuronal vulnerability to oxidative stress in the brain

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Zhang R, Zhang J, Fang L, Li X, Zhao Y, Shi W, An L