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rabbit anti-Hb, 1:500) were applied overnight at 4 C, followed by incubation with secondary antibodies for 2 h in the dark

(PubMed) Andersson M, Aeberli I, Wust N, et al
The mRNA results are presented as means standard errors of the mean (SEM)
Kornmann B, Schaad O, Bujard H, Takahashi JS, Schibler U (2007) System-driven and oscillator-dependent circadian transcription in mice with a conditionally active liver clock

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Most men and women notice an improvement after the first month of taking hormone shots