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The Clock Starts Now: The Art of Reconstitution This is the moment of truth

For example, Sharafkhah et al

Cells were washed twice in PBS + 1% FBS and resuspended in 200 L of 7-AAD viability dye diluted 1:200 (Invitrogen, #A1310)

Are there any contraindications in my medical history I should be aware of

Pollen deprivation for long periods, starting from 7 days, leads to statistical significant decreases in protein concentrations in worker bees [9, 10]
